Figure 9. p53, Apaf1, Caspase3 and Caspase7 gene expression after stress treatment.

The gene express pattern in the testis of Cynops orientalis after cold exposure at (4°C 4 h and 12 h) and heat exposure (38°C 2 h, 12 h and 24 h; 40°C 2 h and 4 h) determined by real-time reverse transcription-PCR. Messenger RNA levels in experimental group were presented as the folds of gene expression level in control group, which was set as 1.0. All data were expressed as Means±SD, and T-test in SPSS9.0 applied to analyze the significance of differences between experimental group and control group. * P<0.05 when compared with control. n  = 3.