Figure 11. Immunofluorescence analysis of spontaneous apoptosis and stress induced apoptosis in newt mature sperms.

The apoptotic germ cells were determined by TUNEL kit (Beyotime, China) and the nucleus were dyed with DAPI. Apoptotic sperms were found in normal newt testis (A1-3), and severer mature sperm apoptosis detected under various stress including starvation (B1-3), cold exposure(4°C for 12 h) (C1-3), cadmium exposure (5 mg/Kg body weight for 36 h) (D1-3) and heat exposure (40°C for 2 h) (E1-3). The scale bar is 10 µm.