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. 2012 Jun 29;7(6):e39920. doi: 10.1371/journal.pone.0039920

Table 1. Primers used for the study of Caspase3 and Caspase7 in Cynops orientalis.

Primer name Primer Sequence (5′to 3′) Usage
Csp AF1 ATTGTATTATTATTAATaayaaraaytt Caspase7/3 cloning
Csp BF2 AAGAAATGGAacngaygtnga Caspase7/3 cloning
Csp DR2 GAATAAGCATACAGAAAATCNGCYTCNAC Caspase7/3 cloning
Csp CR1 CCTGAATGAAAAACAGTTTNGGYTTNCC Caspase7/3 cloning
Csp CF1 ACCAAAACTGTTCTTTATTcargcntgymg Caspase3 cloning
Csp3lF1 AAAGTTTTCAATGACCAAGC Caspase3 cloning
Csp3lR1 ATCTGACGAATCTCCTCCAC Caspase3 cloning
Csp7-3′gspF1 CTTATCTATGGGACAGACGGGC Caspase7-3′RACE
Csp7-3′gspF2 GGTGAAGAGGGACTTATCTATGGG Caspase7-3′ RACE
Csp7-5′gspR1 GCTGCTCCATTTCTTCACAGGTCCGA Caspase7-5′ RACE
Csp7-5′gspR2 AGCAGGCATAATCACTGTGGTCTTCTT Caspase7-5′ RACE
Csp3-3′gspF1 ATCAACAGAGGATACCCGTGGA Caspase3-3′ RACE
Csp3-3′gspF2 GCAGACAGTGGAGGAGATTCGT Caspase3-3′ RACE
Csp3-5′gspR1 GTAAGAAAACACTTCCTGAGATTTGCGG Caspase3-5′ RACE
Csp3-5′gspR2 CCCAAGGTAAGAAAACACTTCCTGAGAT Caspase3-5′ RACE
Csp7rlF1 TGGATGTTTATGTCACCTTTTC Realtime PCR
Csp7rlR1 AGTCTATTTTATTATGGGCTCG Realtime PCR
Csp3rlF1 AGAGAACAATGGCGGATA Realtime PCR
Csp3rlR1 CCAGTTGAGGGATGAAAG Realtime PCR
R(A,G) Y(C,T) M(A,C) N(A,T,G,C)