Table 2. Primers used for the study of p53 and Apaf1 in Cynops orientalis.

Primersname	Primer Sequence(5′to 3′)	Usage
P2F1	TGAGAAGATGTCCACAYCAYGARMG	p53 Cloning
P5R1	CAAGCACACACTCKNACYTCRAA	p53 Cloning
P5R2	ATCTCTTCCTGGACANGCRCANAC	p53 Cloning
P5′-gspR3	CCTGCGGAGTCTCGTATGGCACCAACAC	p53 5′ RACE
P5′-gspR2	TGGCACCAACACGCTGTGGCGACGGG	p53 5′ RACE
P3′-gspF3	GCCACAGCGTGTTGGTGCCATACGAGAC	p53 3′ RACE
P3′-gspF2	CGCCGACCAATCCTGACTATCATTACAC	p53 3′ RACE
P53RTF1	CCCCCAAGAATGGCAAAAA	RT-PCR
P53RTR1	CCTGGCTCACCCCCACCTA	RT-PCR
p53rlF1	GATGACTGTCTCCACCCCT	Realtime PCR
p53rlR1	TCTGCCTTGTAATTCCCTT	Realtime PCR
β-actinF	GAATCCCAAAGCCAACC	Realtime PCR/RT-PCR
β-actinR	CCAGCCAAGTCAAGACG	Realtime PCR/RT-PCR
Apaf2F	TTATGAAGCTCTGGAYGARGCNAT	Apaf1 cloning
Apaf4R2	TTTAAAAATCTGCAGTGTTTTRTCNGCNCC	Apaf1 cloning
Ap3′-gspF1	ATGCTTGCTTTTCCCCTGATGG	Apaf1 3′ RACE
Ap3′-gspF2	GTCCGCATACAGATGGAGTTTA	Apaf1 3′ RACE
Ap5′-gspR1	GCATTTCTTCCACCTCTTCTGTCTCCA	Apaf1 5′ RACE
Ap5′-gspR2	GTGAGAAAATCCAGTTGAAGGTCGTGT	Apaf1 5′ RACE
AprlF1	ATGGAGTTTATCATGCTTGC	Realtime-PCR/RT-PCR
AprlR1	CATAGGATTAGTTCCGTTGG	Realtime-PCR/RT-PCR
R(A,G) Y(C,T) M(A,C) K(G,T) N(A,T,G,C)