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. 2012 Jun 29;7(6):e40027. doi: 10.1371/journal.pone.0040027

Figure 8. Disulfiram inhibits dimerization of MBP-eAPP230–624.

Figure 8

A) Small angle x-ray scattering analysis of MBP-eAPP230–624 incubated with 50 µM disulfiram or 50 µM sulfiram at a 1∶12 molar ratio in PBS plus 2% DMSO. Incubation with either disulfiram or sulfiram produces significant deviations in the scattering curves indicating a significant shift in size and shape. The Guinier plot of MBP-eAPP230–624 (black), sulfiram-treated (blue), and disulfiram-treated (red) is shown in the inset. The y-axis of the Guinier plot is normalized so that one unit is proportional to 90-kDa (the molecular mass of MBP-eAPP230–624). The molecular mass of the complex can be obtained from the extrapolated y-intercept of the best-fit line. The downward shift of the line indicates that both sulfiram and disulfiram shift the monomer-dimer equilibrium in favor of the monomer. The curvature of the Guinier plot for disulfiram is evidence that the sample is a mixture of monomer and dimer. B) Cleavage of MBP-eAPP230–624 with ADAM10 as measured by AlphaLisa.