Skip to main content
. 2012 Jun 29;7(6):e39956. doi: 10.1371/journal.pone.0039956

Figure 2. Expression of BER proteins in monocytes and follow-up during differentiation of monocytes in DCs and macrophages and BER activity in monocytes.

Figure 2

Expression of PARP-1, XRCC1 and ligase IIIα during maturation of monocytes into DCs (A) and macrophages (B) analyzed by immunoblots. (C) Expression of PARP-1, XRCC1 and ligase IIIα in monocytes, DCs and macrophages without treatment and 24 and 48 h following treatment with 0.6 mM TMZ. (D) BER assay in untreated and TMZ treated monocytes 24 and 48 h post-treatment. The 39mer fragment represents the full-length oligonucleotide. The 19mer and the 19+1 fragment are products of the initial incision and processing, respectively. Restoration of the 39mer fragment represents the re-ligation step. For positive, repair competent, control we used extracts of DCs. (E) Quantification of the full-length fragment shown in (D). The relative amount of the 39mer is shown as a function of time following TMZ.