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. 2012 Jun 29;7(6):e39956. doi: 10.1371/journal.pone.0039956

Figure 5. Activation of ATM, ATR, p-H2AX, Chk1, Chk2 and p53 in monocytes.

Figure 5

(A) Western Blot analysis of DDR proteins and p53 in monocytes not treated (zero time) and treated with 0.6 mM TMZ. (B) Quantification of the subG1 fraction in monocytes co-treated with 0.6 mM TMZ and the indicated kinase inhibitors for 72 h. (C) Western Blot analysis of p53 activation in monocytes co-treated with 0.6 mM TMZ and kinase inhibitors as indicated for 48 h. Cells were pretreated with 10 µM wortmanin, 10 µM Ku55933, 10 µM Chk1 and 10 µM Chk2 inhibitor for 1 h prior to TMZ addition. Cells were post-treated with 10 µM Ku55933, 10 µM Chk1 and 10 µM Chk2 inhibitor every 24 h following TMZ treatment.