Skip to main content
. 2012 Jun 29;7(6):e40246. doi: 10.1371/journal.pone.0040246

Figure 1. The expression changes and sequence of PsMYB1.

Figure 1

(A) Overview of expression changes during two stages of PsSAK1-silenced strain T31. Illustrated are the stages examined (cysts and 1.5 h after inoculation onto susceptible soybean leaf tissues) and number of up- or down-regulated genes during each stage. (B) Alignment of PsMYB1 orthologs. The R2 and R3 Myb DNA-binding domains are underlined by red and blue lines, respectively. The orthologs are from P. infestans (PITG_06748.1), P. ramorum (Pr_86538), H. arabidopsidis (Ha_811065), Pythium ultimum (PYU1_T010492), and Albugo laibachii (A. laibachii Nc 14). (C) Validation of DGE data by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). PsMYB1 transcripts in the PsSAK1-silenced line are down-regulated dramatically in cysts stage and IF1.5 h stage based on the qRT-PCR. Results of an independent-samples t-test for each species. *P<0.05; **P<0.001.