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. 2012 Jun 29;7(6):e40246. doi: 10.1371/journal.pone.0040246

Figure 2. qRT-PCR assay of the PsMYB1 gene.

Figure 2

(A) Expression pattern of PsMYB1 based on qRT-PCR. The ten stages are: mycelia (MY), sporulating hyphae (SP), zoospores (ZO), cysts (CY), germinating cysts (GC), and IF1.5 h to IF24h (samples from 1.5, 3, 6, 12, and 24 h after infection of soybean leaves). (B) Expression of PsMYB1 in strains transformed with extra copies of PsMYB1. qRT-PCR was performed using sporangiating hyphae RNAs from wild-type isolate (P6497) and transformants (PsMYB1 silenced, T54, T77, and T101; PsMYB1 non-silenced, T132) as initial template. (C) PsSAK1 expression level in the PsMYB1-silenced line. qPCR was performed based on the ΔΔCT method. Error bars are calculated from three replicate qRT-PCR assays.