Figure 5. Analysis of PsMYB1 gene expression during sporangial direct germination.

The RNAs of sporulating hyphae were prepared by repeatedly washing 2-day-old hyphae incubated in 10% V8 broth with sterile distilled water and incubating the washed hyphae in the light at 25°C for 4–8 h until sporangia developed on most of the hyphae. "M+", washing the P6497 hyphae with sterile distilled water containing 0.8 M mannitol and induce the sporangia of P6497 to germinate directly. "CK", washing mycelia of the wild-type with sterile distilled water and induce the sporangia of P6497 to germinate indirectly. Evaluating expression of PsMYB1 in P6497 sporangia of germinated indirectly and that of germinated directly. Expression was measured by quantitative real time PCR and normalized to the level in the sporangia of germinated indirectly of wild-type P6497. Bars indicate standard errors from three independent replicates.