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. 2012 Jun 29;7(6):e40355. doi: 10.1371/journal.pone.0040355

Figure 4. Effect of cryptopleurine on the TNF-α-induced NF-κB-dependent reporter gene expression.

Figure 4

(A) MDA-MB231 cells were transiently transfected with a NF-κB reporter construct pNF-κB-Luc for 24 h. After transfection, cells were incubated with indicated concentrations of cryptopleurine for 12 h and then treated with 20 ng/ml TNF-α for an additional 12 h. The lysates of MDA-MB231 cells were subject to the measurement of dual luciferase activity. Data represented as mean ± standard deviation of three independent experiments. **p<0.01, ***p<0.001, significantly different when compared with TNF-α-stimulated normal cells. (B) MDA-MB231 cells were transiently transfected with a NF-κB reporter construct pNF-κB-Luc alone or with plasmids expressing the indicated proteins. After transfection, cells were incubated with 30 nM cryptopleurine for 12 h and then incubated with the relevant plasmid for an additional 12 h. TNF-α-treated cells were incubated with 30 nM cryptopleurine for 12 h and then treated with 20 ng/ml TNF-α for an additional 12 h. The lysates of MDA-MB231 cells were subject to the measurement of dual luciferase activity. Data represented as mean ± standard deviation of three independent experiments. ***p<0.001, significant with respect to control.