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. 2012 Jun 29;7(6):e40355. doi: 10.1371/journal.pone.0040355

Figure 7. Effect of cryptopleurine on the TNF-α-induced invasion activity.

Figure 7

(A) MDA-MB231 cells were incubated with 30 nM cryptopleurine for 12 h and then incubated with 20 ng/ml TNF-α for the indicated times. Whole cell extracts were analyzed by Western blot using indicated antibodies for ICAM-1, MMP-9, VEGF, and tubulin. (B) MDA-MB231 cells invaded through the pores in the Matrigel-coated filters were fixed, stained and counted in five random field visualized by microscopy (100×). Data represented as mean ± standard deviation of three independent experiments. ***p<0.001, significant with respect to control.