Figure 6. No effect of adult-onset hypothalamus-specific Y1Y5 receptor deletion on energy homeostasis on a chow diet.

(A–H) Spontaneous (A, C, E, G) and accumulated (accum) 24-hour fasting-induced food intake (B, D, F, H), normalised to body weight, in adult-onset hypothalamus-specific Y1Y5 receptor deficient (Y1Y5^Hyp/Hyp) or control (Y1Y5^lox/lox) mice at 18 and 34 weeks of age. (I–J) Body weight from 12 to 36 weeks of age in male (I) and female (J) Y1Y5^Hyp/Hyp or Y1Y5^lox/lox control mice. ‘PVN injection’ refers to the time of induction of gene deletion by injection of a Cre-recombinase-expressing viral vector into the paraventricular nucleus of the hypothalamus. (K–P) Fat and lean masses as a percentage of body weight (%BW), measured using dual energy x-ray absorptiometry, in male (K, O) and female (M, P) Y1Y5^Hyp/Hyp and Y1Y5^lox/lox mice at 19, 27 and 36 weeks of age. Weight (as %BW) of dissected white (WAT) and brown adipose tissue (BAT) depots at the end of the study in male (L) and female (N) Y1Y5^Hyp/Hyp and Y1Y5^lox/lox mice at 36 weeks of age. Abbreviations: i, inguinal; g, gonadal; m, mesenteric; r, retroperitoneal; total, summed weight of i, g, m and r WAT depots. Plotted values are means ± SEM of 5–12 standard chow-fed mice per group.