Table 8. Primers for 14 target genes and β-actin gene used for Real-time PCR.

Gene (contig number)	Primer	Primer sequence (5′-3′)
myosin	myosin_F36	GGAAAGTTCAGTGTTCTCCGTCAT
(NODE_94559)	myosin_R170	TACGAAACAGGTTAGACTCCAAAG
glucose transporter	glu-tra_F28	TGCGTGAGTGACGGTGGAGATGTG
(NODE_48440)	glu-tra_R199	ACATCCACCTCTGCCAGCAGATG
monoamino oxidase (MONO)	MONO_F1170	GGGCGGAAGAACAATACAGTGGA
(NODE_2713)	MONO_R1453	AAAGAAGGTCTCATCGAAAGGAC
tight junction protein 1 (ZO-1)	ZO_F55	TCCTTCTGGTAGAGCACCTTTT
(NODE_84454)	ZO_R210	CTGGAAACAGACCACGAAAAAT
alpha-catenin	A-catenin_F640	GGCGACAATGATGCTGCTAACTT
(NODE_17600)	A-catenin_R798	AGGCTGTCACTAGACACTCCACCA
beta-catenin	B-catenin_F1262	GGACTACTTCCAGAGTACTTAAAGT
(NODE_2995)	B-catenin_R1408	TTCTCAGAGTCCAGAGGCAGTTAT
Catechol-O-methyltransferase (COMT)	COMT_F137	ATGGGCAGGTCAAGTCCTCAAGAT
(NODE_96469)	COMT_R348	TGATGGTGATTTTCTTGCCATGTG
Aromatic L-amino acid decarboxylase (AAAD)	AAAD_F222	GCCTCATACCATTTTTCGTTTGTG
(NODE_49080)	AAAD_R395	CGCCATTCAGTAGGGGTCGGTATT
Multidrug resistance-associated protein (MRS)	MRS_F217	GAGGGAATCAGTTTCCGCATCAAC
(NODE_85752)	MRS_R430	CAGATGAACAAATATGGTCGGCACT
P-gp	Pgp_F193	TGCTAATGACGCTTGGATGTTTCT
(NODE_10911)	Pgp_R360	CGTTATGATACGCTTGTTGGTGAA
cingulin	cingulin_F590	ATTCTTCCTTCTCACCAGCCTCAC
(NODE_5285)	cingulin_R771	AGAAATCACTCGGTTAAACACTC
Junctional adhesion molecule-1 (JAM)	JAM_F144	CCTTCAACAGGCTCACTCACTTCT
(NODE_6101)	JAM_R375	CTCCCAAAGTTCAGTGGAGATACA
Organic anion transporter (OAT)	OAT_F122	CATAGGCTTAGCAGAGTTTCCAG
(NODE_79656)	OAT_R292	GTCAAAGCGATAGTAAACCCAAT
N-cadherins	cadherins_F300	TGACCCTACCATTCCGTATCACAA
(NODE_4866)	cadherins_R443	CCACCACATTGGCAGTGTTGTTC
β-actin	β-actin_F1	TGATGGCCAAGTTATCACCA
	β-actin_R1	TGGTCTCATGGATACCAGCA