Figure 3. More calumenin is bound onto G551D-CFTR than onto Wt-CFTR.

A, Example of detection of calumenin in Wt-CFTR and G551D expressing cells, assessed by western blotting using whole cell lysates. No difference was observed between the two cell lines. Pure Calumenin was loaded as a molecular weight control because of the presence of non-specific bands on the membrane. B, Example of coimmunoprecipitation showing the calumenin-CFTR interaction in total proteins (left). Histogram represents the quantitation indicating that more calumenin is bound onto G551D-CFTR than onto Wt-CFTR (right).