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Total RNA was extracted from LV tissue with TRIzol reagent. Expressions of kallikrein 1, 2, 10 and BK2 receptor and ACE 2 mRNA in normalized to GAPDH at 4 and 15 wk ACF rats compared to age-matched shams. Values are mean±SEM. n = 6–8 in each group. *P<0.05, **P<0.01 and ***P<0.001 vs. age-matched shams.
