Skip to main content
. 2012 Jun 11;109(26):10486–10491. doi: 10.1073/pnas.1202607109

Fig. 1.

Fig. 1.

Systemic pretreatment with poly(I:C) suppresses CD8 T-cell clonal expansion and Ag-dependent effector responses. (A) C57BL/6 mice were injected with PBS or 1 μg poly(I:C) on day −3, with OT-I cells on day −1, and with 50 μg SIINFEKL peptide on day 0. The numbers of tissue OT-I cells were enumerated on day 3. (B) C57BL/6 mice were injected with PBS or 1 μg poly(I:C) on day −3 followed by 1 μg SEA on day 0 or SEA together with poly(I:C) on day 0. Splenic Vβ3+ cells were enumerated on day 3. (C) C57BL/6 mice were injected with 100 μg poly(I:C) or PBS on day −3 and immunized with 100 μg SIINFEKL peptide, 50 μg CpG, and 5 μg anti–4-1BB agonist antibody on day 0. Splenic SIINFEKL-tetramer+ cells were analyzed on day 6. (D) Phenotypes of splenic OT-I cells from A. (E) Splenocytes from A were restimulated with SIINFEKL peptide to determine the frequency of effectors. (F) Ex vivo granzyme B expression on OT-I cells in A. Data are representative of three to four experiments.