Fig. 2.

Poly(I:C) pretreatment inhibits IL-33 receptor (ST2) up-regulation on CD8 T cells during primary responses and after restimulation. C57BL/6 mice were injected with PBS or 50 μg poly(I:C) on day −3 and with OT-I cells on day −1 and immunized with SIINFEKL peptide on day 0. (A) Splenocytes were harvested on day 3, and ST2 expression was analyzed by gating on total CD8 T cells. (B) Splenocytes were restimulated ex vivo for 3 h, and ST2 expression on OT-I was analyzed by gating on total CD8 T cells. Results were obtained from three to four experiments.