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. 2012 Jun 11;109(26):10486–10491. doi: 10.1073/pnas.1202607109

Fig. 3.

Fig. 3.

Poly(I:C) pretreatment impairs CD8 T-cell responses to IL-33. (A and B) C57BL/6 mice were injected with PBS or 50 μg poly(I:C) on day −3 and with OT-I cells on day −1 and immunized with SIINFEKL peptide on day 0. Lymph nodes were harvested on day 3, and OT-I cells were purified by magnetic beads (A) or FACS sorting (B) before a 24-h culture with different cytokines. (C) OT-I CD8 T cells were activated in vivo by SIINFEKL peptide and purified from lymph nodes 3 d post immunization. IFNγ release by OT-I CD8 T cells in response to log-fold titration of IL-33 and IL-12 was measured. (D and E) OT-I CD8 T cells were activated in vivo by SIINFEKL peptide, anti–4-1BB, and anti-OX40 agonist antibodies. OT-I cells were purified from lymph nodes 3 d post immunization and used for ex vivo culture. UD, undetected. Data shown were obtained from at least three independent experiments.