Fig. 4.

Recursive multiplex recombineering hydrolysate selection isolates. (A) Relative growth over 44 h of 10 isolated clones taken from sample plates after the initial selection. (B) Mutations in RBS of tolerant clones. (C) Relative growth over 20 h of four mutants isolated after the second selection compared with WT and H1 G and I (isolated from the first selection). (D) Mutations in RBS of the second combinatorial library clones isolated after the second selection. Error bars represent 1 SD.