Fig. 2.
Migration speed versus ECM stiffness and channel width. (A) Migration speed versus channel width for soft, intermediate and stiff ECMs, corresponding to E = 0.4, 10, and 120 kPa. *p < 0.05 with respect to narrow (cw = 10 μm) channels. (B) Migration speed versus ECM stiffness for narrow, intermediate, and wide channels (cw = 10, 20, and 40 μm) and flat 2D gels. *p < 0.05 for pairwise comparison between two indicated stiffness values for all given channel widths, including 2D flat gel. n > 35 cells per condition over multiple channels and devices. (C) Phase contrast images of cells migrating inside channels of varying stiffness and width. Scale bar = 40 μm.