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Morphological polarization of U373 cells on flat 2D gels. (A) Cell polarization measured as aspect ratio of cells cultured on flat unconfined (2D) gels. *p < 0.05 for indicated pairwise comparison; n > 35 cells per condition. (B, C, D) Phase contrast images of cells on 2D flat gels of varying stiffness. Scale bar = 40 μm.
