Fig. 5.
Effect of nonmuscle myosin II inhibition on cell motility. (A) Migration speed versus ECM stiffness for varying channel width. n > 30 cells per condition. (B) Phase contrast images of cells inside narrow and wide channels and flat 2D gels of varying stiffness. Scale bar = 40 μm. (C) Confinement sensitivity, Δν, calculated as |νnarrow - νwide|, where νnarrow and νwide are average respective migration speeds for narrow (cw = 10 μm) and wide (cw = 40 μm) channels versus ECM stiffness for control and blebbistatin-treated cells. Statistical significance (*p < 0.05) determined by Student’s t test (unpaired, two-tailed, 95% confidence interval).