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. 2012 Jun 11;109(26):10382–10387. doi: 10.1073/pnas.1206468109

Fig. 3.

Fig. 3.

Gadkin colocalizes with a subpool of ARP2/3 on endosomes. (A–D) Gadkin and ARPC2 colocalize under conditions that do not stimulate lamellipodia formation. Localization of Gadkin-EGFP and ARPC2 in B16F1 cells grown on Matrigel (A) or on laminin in the presence of aluminum fluoride (C) or in the presence of Rac1 inhibitor (E). Twenty-four hours after seeding, cells were fixed and immunostained with ARPC2-specific antibodies. (B and D) Fluorescence-intensity profiles along lines indicated within boxed areas in (A and C). (F) Pearson correlation coefficients illustrating the degree of colocalization between Gadkin-EGFP and ARPC2 under different conditions (40–67 cells per condition from 2 to 4 experiments; error bars represent SEM; **P < 0.01; one-way ANOVA plus Dunnett’s post test). (G and H) Gadkin-EGFP-expressing B16F1 cells were treated with Rac1 inhibitor. Twenty-four hours after seeding, cells were fixed and costained with antibodies specific for the indicated protein. (Scale bar: 5 μm.) (Insets) Magnifications: 5× (A–E); 4× (G and H).