Fig. 1.
Binding of P. falciparum-IEs to primary HBMECs. (A) Parasites exhibiting different binding phenotypes were compared for binding to resting or TNF-α–activated HBMECs from patient donor 13. The A4longHBMEC and ItG-ICAM-1HBMEC lines were panned three times on HBMECs from donor 13. Results are expressed as mean number of IEs per square millimeter ± SDs from two to three independent experiments (***P < 0.001). (B) The starting A4long and ItG-ICAM-1 parasite lines exhibited a concentrated binding to a subpopulation of HBMECs that became more dispersed after panning of IEs three times on HBMECs.