Fig. 3.

HBMEC-panned IEs adhere to brain microvascular endothelial cells from different patient sources. (A) Binding was compared between the starting (ItG-ICAM-1) and selected (ItG-ICAM-1^HBMEC) parasite lines on HBMECs harvested from donors 13, 56, and 123 and a transformed HBMEC line (THBMEC) from donor 13. The anatomic origin of HBMEC cultures is illustrated in the brain schematic. (B) A DC8-var-expressing clonal parasite line derived from ItG-ICAM-1^HBMEC bound to primary microvascular cells from heart, lung, and dermis.