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. 2012 May 22;109(26):E1782–E1790. doi: 10.1073/pnas.1120534109

Fig. 4.

Fig. 4.

New var genes are up-regulated in parasite lines panned on primary HBMECs. (A and B) Transcription of var genes was compared from ring-stage parasites before and after panning on primary HBMECs (donor 13). Results were normalized to the housekeeping control gene adenylosuccinate lyase (asl). Genes are organized by Ups category, UpsA (red), UpsB (blue), UpsC (yellow), and UpsE (gray), as well as three genes for which the Ups type has not been determined (white). The names of parasite lines are indicated at the left, and var genes that expressed onefold or more of asl are indicated. (C) The extracellular domain architecture and predicted binding features of the three main genes up-regulated on HBMECs are shown. The DC8 cassette in IT4var6 and IT4var19 and the DC17 cassette in IT4var13 are underlined. The percentage of amino acid domain identity is shown for the two DC8-var products. Binding predictions are from repertoire-wide analysis of PfEMP1 recombinant proteins between CIDR-CD36 (14) and DBLβ-ICAM-1 (38).