Fig. 7.
Endemic sera recognition of HBMEC-panned parasite lines. (A) Acute and convalescent sera pairs were collected from 10 children with cerebral malaria and 10 age-matched children with nonsevere malaria. (B) Surface recognition of infected erythrocytes was analyzed by flow cytometry. The mean fluorescence intensity (MFI) of uninfected erythrocytes was subtracted from the MFI of the infected erythrocyte population to give a specific MFI of the infected erythrocytes. The specific MFI reported in the graph was further corrected by subtracting antibody reactivity of a nonimmune European control plasma, which had an MFI of 1 on ItG-ICAM-1HBMEC and no reactivity on ItG-ICAM-1. Antibody reactivity between starting and selected parasite lines was significantly different at the time of acute disease and at convalescence (***P < 0.001, Mann–Whitney test).