Fig. 2.

Identification of full-length sequences of dominant var transcripts in one patient with cerebral malaria (1914), one patient with cerebral malaria and a hemoglobin level of 5 g/dL (1983), and one patient with severe anemia without cerebral symptoms (1965). Bar charts show proportions of specific DBLα tag reads relative to the total read number (gray bars/left y axes) and the transcript level (T_u) measured by RT-qPCR using primers specific for the targeted genes (white bars/right y axes). Colored panels show domain and DC annotation of full-length genes identified from the DBLα tag analysis. Gene numbers correspond to the DBLα tag numbers (bar charts, x axes). Domains marked by numbers were found by in silico analysis to be targeted by correspondingly numbered primers reporting high transcript levels, defined as T_u > 8 units (corresponds roughly to half the level of seryl-tRNA-synthetase). The high-abundance primers are listed below domain annotations according to T_u with the highest T_u (1 = highest T_u). Primers in bold are predicted to amplify sequences in the annotated full-length genes, and primers in italics are not.