Fig. 3.

WGBS data validation with a 450-K CpG site DNA methylation microarray. (A) Comparison of CpG methylation differences between the NB and Y103 obtained from WGBS (x-axis) and 450-K array (y-axis) technology. Displayed are all differentially methylated CpG sites derived from the WGBS approach that were also present on the 450-K array (3,205). The threshold of 0.20 change in β-values is indicated (broken line). (B) The curves of the distance correlation between the methylation status of the neighboring CpG sites of NB (blue line) and Y103 (red line) samples determined by the 450-K microarray. A more pronounced declining curve indicates lower correlation in terms of the methylation status of nearby CpGs.