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. 2012 Jun 11;109(26):10474–10479. doi: 10.1073/pnas.1114214109

Fig. 1.

Fig. 1.

RhoH negatively regulates chemokine-mediated Rap1 activation and positively regulates TCR-mediated Rap1 activation. (A–C) Jurkat cells were transiently transfected with mRFP or RhoH–mRFP, then at various time after stimulation with CXCL12 or CD3 cross-linking, cell lysates were assessed for active Rap1, total Rap-1, and b-actin. Representative blots of Rap1 activation by CXCL12 (A) or CD3 cross-linking (B) are shown and bar graphs of each pair of blots consist of compiled densitometry analysis (Right). (C) Freshly isolated CD4+ T cells from WT and RhoH−/− mice were unstimulated (NS) or stimulated with CD3 cross-linking or CCL21 and Rap1–GTP assessed. Data are means ± SEM, n = 3, and statistically significant, *P < 0.05 vs. WT NS, statistically significant, #P < 0.03 vs. RhoH−/− CD3.