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. 2012 Jun 29;7(6):e40144. doi: 10.1371/journal.pone.0040144

Figure 1. A CK2 inhibitor inhibited ER stress-induced GRP78 expression.

Figure 1

4,5,6,7-tetrabromobenzotriazole (TBB) inhibited GRP78 expression at the mRNA and protein levels. (A) Primary cultured glial cells were pre-treated with 4,5,6,7-tetrabromobenzotriazole (TBB: 5 µM) for 3 h and then treated with tunicamycin (Tm: 0.01 µg/mL) or thapsigargin (Tg: 0.01 µM) for 6 h. RT-PCR was performed using specific primers for each mRNA. n = 4/group *p<0.05 compared with ER stress (Tm, Tg) alone. (B) Primary cultured glial cells were pre-treated with 4,5,6,7-tetrabromobenzotriazole (TBB: 5 µM) for 3 h, then treated with tunicamycin (Tm: 0.01 µg/mL) or thapsigargin (Tg: 0.01 µM) for 18 h, and subjected to Western blotting. n = 3/group *p<0.05 compared with ER stress (Tm) alone. Results are expressed as the means ± S.E.