Skip to main content
. 2012 Jun 29;7(6):e40144. doi: 10.1371/journal.pone.0040144

Figure 2. Knocking down CK2 inhibited ER stress-induced GRP78 expression.

Figure 2

(A) Western blot analysis of endogenous CK2α expression in lysates of nontransfected cells (NT) and cells transfected with (short interfering RNA) siRNAs (75 nM) directed at CK2 or the control sequence. CK2 siRNA reduced the expression of CK2 compared with NT or control siRNA. (B) CK2 siRNA decreased ER stress-induced GRP78 expression compared with control siRNA. Primary cultured glial cells were transfected with 75 nM siRNA and treated with tunicamycin (Tm: 0.01 µg/mL) for 4 h. RT-PCR was then performed. n = 3/group (C, D) CK2 siRNA decreased ER stress-induced GRP78 expression compared with control siRNA. Primary cultured glial cells were transfected with 75 nM siRNA, treated with tunicamycin (Tm: 0.01 µg/mL) or thapsigargin (Tg: 0.01 µM) for 18 h, and subjected to Western blotting. n = 3–4/group *p<0.05, **p<0.01 compared with control siRNA. Results are expressed as the means ± S.E.