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. 2012 Jun 29;7(6):e40144. doi: 10.1371/journal.pone.0040144

Figure 3. The CK2 inhibitor inhibited ER stress-induced XBP-1 mRNA splicing.

Figure 3

(A) Primary cultured glial cells were pre-treated with 4,5,6,7-tetrabromobenzotriazole (TBB: 5 µM) for 3 h, treated with tunicamycin (Tm: 0.01 µg/mL) or thapsigargin (Tg: 0.01 µM) for 6 h, and subjected to a RT-PCR analysis. (B) Densitometric analysis of spliced/unspliced XBP-1 mRNA. An ER stress inducer increased XBP-1 splicing and this effect was significantly inhibited by TBB. n = 4–5/group *p<0.05 compared with ER stress (Tm, Tg) alone (C) Densitometric analysis of total XBP-1 mRNA (unspliced and spliced XBP-1 mRNA). n = 5/group. TBB did not affect the XBP-1 transcript. Results are expressed as the means ± S.E.