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. 2012 Jun 29;7(6):e39302. doi: 10.1371/journal.pone.0039302

Figure 5. L-NAME treatment increased neurogenesis and seizure susceptibility.

Figure 5

Daily treatment with L-NAME for 24 days (A) resulted in a significant increase in the number of fully seizing animals after the second PTZ administration on day 25 (B). In control animals, nestin immunoreactivity was detected in capillary walls (C). By quantitative RT-PCR there was a 7.8-fold (p = 0.001) in nestin mRNA levels at day 3 post-seizure (D). L-NAME-treated animals had significantly more (2.7-fold; p = 0.001) nestin mRNA than did animals treated with PTZ alone at day 25 (D) whereas kindled animals did not show an increased level of nestin mRNA (D). At the tissue level, nestin immunoreactivity at day3 in PTZ-treated animals was confined to radial glia-like cells in the inner molecular layer of the dentate gyrus, the polymorphic layer and, interestingly, to the CA2 region (arrow) (E, and inset). After 25 days, the nestin-like immunoreactivity was restricted to the polymorphic layer (F and inset). Abbreviations: DG, dentate gyrus; pml, polymorphic layer; CA2, hippocampal region.