Table 2. Sequences of primers and probes used in the study.

Name	Sequence 5′-3′	Genome position*	Reference
PRRSV-US-1dF	ATRATGRGCTGGCATTC	15257–15273	Kleiboeker et al., 2005, modified
PRRSV-US-1R	ACACGGTCGCCCTAATTG	15353–15370
PRRSV-US-1TEX	ACACGGTCGCCCTAATTG	15307–15330
PRRSV-HP-1F	CCGCGTAGAACTGTGACAAC	2914–2933	this study
PRRSV-HP-1R	TCCAGGATGCCCATGTTCTG	3035–3016
PRRSV-HP-1Cy5aS	Cy5-ACGCACCAGGATGAGCCTCTGGAT-BHQ3	2941–2964
PRRSV-EU-2.1F	GCACCACCTCACCCRRAC	14792–14809	Kleiboeker et al., 2005, modified
PRRSV-EU-2.1R	CAGTTCCTGCRCCYTGAT	14851–14868
PRRSV-EU-2.1FAM	FAM-CCTCTGYYTGCAATCGATCCAGAC-BHQ1	14819–14842
EGFP-11-F	CAGCCACAACGTCTATATCATG	537–558	Hoffmann et al., 2006
EGFP-10-R	CTTGTACAGCTCGTCCATGC	813–794
EGFP-HEX	HEX-AGCACCCAGTCCGCCCTGAGCA-BHQ1	703–724

^*genome position according to.

type 1-PRRSV: type 1 prototype strain Lelystad (accession number M96262).

type 2-PRRSV: type 2 prototype strain VR-2332 (accession number U87392).

HP-PRRSV: Porcine respiratory and reproductive syndrome virus strain JXwn06.

(accession number EF641008).

EGFP: cloning vector pEGFP-1 (accession number U55761).