Figure 7. Inhibition of stomatin expression enhanced Panx1-dependent outward whole-cell currents in astrocytes.

Cells were transfected with either stomatin siRNA or scrambled siRNA. A. Effects of scrambled and stomatin siRNA on stomatin mRNA level as determined by real-time PCR (from 3 independent experiments). The asterisk (*) indicates a significant difference compared with scrambled siRNA (p<0.01, paired t-test). B. Averaged current traces in response to a voltage ramp (–60 to +90 mV over 10 sec) showing the effects of siRNA and the specific Panx1 channel inhibitor ¹⁰Panx1. C & D. Comparisons of the peak outward (at +90 mV) and inward (at –60 mV) currents among the different groups. The asterisk (*) indicates a significant difference (p<0.01) compared with scrambled siRNA whereas the pound sign (#) indicates a significant difference (p<0.05) compared with stomatin siRNA (one-way ANOVA with Bonferroni posthoc test).