Table 5. New shuttle vectors constructed during this study.
| Name | Accession# | Promotera | Localizationb | Linearization | Selection |
| pPpB1_S | JQ519685 | AOX1 | Intracellular | Blunt | Zeocin™ |
| pPpB1GAP | JQ519686 | GAP1 | Intracellular | Sticky-end | Zeocin™ |
| pPpB1GAP_S | JQ519687 | GAP1 | Intracellular | Blunt | Zeocin™ |
| pPpB1_Alpha_S | JQ519688 | AOX1 | Secreted | Blunt | Zeocin™ |
| pPpT4 | JQ519689 | AOX1 | Intracellular | Sticky-end | Zeocin™ |
| pPpT4_S | JQ519690 | AOX1 | Intracellular | Blunt | Zeocin™ |
| pPpT4_Alpha_S | JQ519691 | AOX1 | Secreted | Blunt | Zeocin™ |
| pPpT4GAP_S | JQ519692 | GAP1 | Intracellular | Blunt | Zeocin™ |
| pPpT4GAP_Alpha_S | JQ519693 | GAP1 | Secreted | Blunt | Zeocin™ |
| pPpKan_S | JQ519694 | AOX1 | Intracellular | Blunt | KanMX6 |
| pPpKan_Alpha_S | JQ519695 | AOX1 | Secreted | Blunt | KanMX6 |
| pPpARG4 | JQ519696 | AOX1 | Intracellular | Sticky-end | ::ARG4 |
| pPpHIS4 | JQ519697 | AOX1 | Intracellular | Sticky-end | ::HIS4 |
| pPpGUT1 | JQ519698 | AOX1 | Intracellular | Blunt | ::GUT1 |
a
Promoter to regulate the expression of the gene of interest.
b
Localization of the recombinant protein. Vectors aimed for intracellular production can be used for the secretory production by adding a signal sequence.