Figure 3. Effect of ANT ligands on ADP-induced alterations in ΔΨm and ADP-ATP exchange rates in Crangon crangon (A, C) and Palaemon serratus (B, D) mitochondria.

A, B: Time courses of safranine O fluorescence reflecting ΔΨm (% scale). Maximum (100%) polarization is the value of safranine O fluorescence prior to the addition of ADP; minimum (0%) polarization is the value of safranine O fluorescence after the addition of 1 µM SF6947 (omitted from the graph). The length of the arrow representing ΔΨm in the y-axis is equal to a 10% change. C, D: Reconstructed time courses of extramitochondrial [ATP] appearing in the medium upon addition of ADP (where indicated), and effect of mitochondrial inhibitors. Vehicle of BKA (2 mM NH₄OH, traces b), bongkrekic acid (BKA, 20 µM, traces c), or carboxyatractyloside (cATR, 1 µM, traces d) were present prior to the addition of ADP. In trace a no inhibitor was present prior to the addition of ADP. E: Characterization of oxygen consumption of Palaemon serratus mitochondria. Black trace represents a time course of oxygen consumption, by 0. 5 mg Palaemon serratus mitochondria suspended in a 2 ml volume. Grey trace represents the negative time derivative of oxygen concentration, divided by mitochondrial mass per volume. Additions of substances are indicated by arrows. ADP: 0.2 mM. cATR: 2 µM. SF 6847: 1 µM. KCN: 1 mM. Dithion.: Dithionite (added in excess). Substrates were glutamate and malate and succinate (5, 5, and 5 mM). Results shown in all panels are representative of at least 4 independent experiments.