Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jun 29;7(6):e39827. doi: 10.1371/journal.pone.0039827

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Joeris et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Flag-tagged full-length LMP7 (LMP7-Flag), proLMP7 fused to mature β5 (proLMP7mβ5-Flag), full-length β5 (β5-Flag) and proβ5 fused to mature LMP7 (proβ5mLMP7-Flag) were over-expressed in lmp7^−/−Mefs by retroviral transduction (A). The lmp7^−/− Mef lines expressing the four different constructs were either left unstimulated or cultured in the presence of 50 U/ml IFNγ for 4 days. Following cell lysis, the Flag-tagged subunits were precipitated with anti-Flag-M2® agarose. Co-precipitation of the catalytic proteasome subunits β1, β2, LMP2 and MECL-1 with the proLMP7-containing subunits LMP7-Flag (B) and proLMP7mβ5-Flag (C) or the proβ5-containing subunits β5-Flag (D) and proβ5mLMP7-Flag (E), was analysed by Two-colour fluorescent immunoblot analysis. The abundance of each subunit was determined in the input material (i), the supernatant of the immunoprecipitation (SN) and the precipitate (P) for both conditions tested.