Table 2. The EC50 for each CaM construct in activation of eNOS citrulline formation.
Mutants | EC50 (nM) |
WTCaM | 75±10 |
B1Q | 161±14 |
B2Q | 125±12 |
B3Q | 170±16 |
B4Q | 148±8 |
The bar values represent the means ± standard deviation. The mean values were obtained from three independently prepared batches of proteins.