. 2012 Jun 29;7(6):e39851. doi: 10.1371/journal.pone.0039851

Table 3. Table 3. Differential effect of mutating Ca²⁺-binding sites at CaM on enzyme activation among NOS isoforms.

	Cyto c Reduction			Citrulline formation or NO production
	eNOS	nNOS	iNOS	eNOS	nNOS	iNOS
CaMs	(%)			(%)
None	30±4^a	4.2 ^b		2.8±0.8 ^a	0.47 ^b
WT	100±12 ^a	100^b	100^c	100±2^a	100^b	100^c
B_1Q	63±7 ^a	11^b	109^c	75±4^a	<2^b	95^c
B_2Q	67±6 ^a	∼50^b	64^c	94±6^a	26^b	41^c
B_3Q	99±11 ^a	76^b	67.7^c	105±9^a	82^b	43^c
B_4Q	55±7 ^a	∼53^b	42.8^c	111±13 ^a	30^b	90^c
B_12Q	34±4 ^a	∼6^d	140^d	24±6^a	∼5^d	75^d
B_34Q	38±5 ^a	∼12^d	100^d	18±5^a	∼10^d	115^d
B_1234Q	27±3 ^a	∼11^d	<25^d	2.3±0.4 ^a	<2^d	<25^d

This manuscript: The activity was obtained in the presence of 0.5 μM CaM concentration. Under these conditions, the activities for eNOS bound to wild-type CaM were ∼14 min⁻¹ min⁻¹ for citrulline formation, and ∼286 min⁻¹ for cytochrome c reduction.

Data from Ref. 21.

Data from Ref. 22.

Data from Ref. 23.

Table 3. Table 3. Differential effect of mutating Ca2+-binding sites at CaM on enzyme activation among NOS isoforms.

Table 3. Table 3. Differential effect of mutating Ca²⁺-binding sites at CaM on enzyme activation among NOS isoforms.