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. 2012 Jun 29;7(6):e39641. doi: 10.1371/journal.pone.0039641

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Kuo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Time dependent [³H]-E3S uptake at pH 5.5 by MDCKII-pcDNA and MDCKII-Oatp4c1 cells during incubation with 0.5 µM [³H]-E3S (A). Time dependent Oatp4c1 mediated uptake of [³H]-E3S at pH 4.5, 5.5 and 7.4 (B). Concentration dependent [³H]-E3S uptake in MDCKII-pcDNA and MDCKII-Oatp4c1 cells after incubation at pH 5.5 for 1 min (C). Concentration dependent Oatp4c1 mediated uptake (1 min) of [³H]-E3S at pH 4.5, 5.5 and 7.4 (D). GF: GF120918, MTX: methotrexate. Each point represents the mean ± S.D. of triplicates. *p<0.05, significant differences from control.