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. 2012 Jun 29;7(6):e39732. doi: 10.1371/journal.pone.0039732

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Greco et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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miRNA (blue) and mRNA (red) expression patterns were determined in DM2 and CTR, and significantly modulated miRNAs and mRNAs were identified. Validation was performed by qPCR for both miRNAs and mRNAs in 13 DM2 patients and 13 CTR. miRNA targets among the mRNAs identified by Class Comparison analysis were predicted by dedicated softwares. Only miRNA/mRNA couples displaying inverse significant correlation were selected and then analyzed by Ingenuity Pathway Analysis software that allowed to identify enriched molecular pathways and functions.