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. 2012 Jun 29;7(6):e39732. doi: 10.1371/journal.pone.0039732

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Greco et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Representative stainings of transverse sections of frozen DM2 muscle biopsies. Top panel shows a hematoxylin and eosin staining; bottom panel shows an immunostaining for fast myosin heavy chain. Atrophic (blue arrows) and hypertrophic (asterisks) myofibers, as well as nuclear clumps (red arrows) and internal nuclei (black arrows) are highlighted. B) Spearman’s correlation between DM2 miRNAs score and Atrophy (AR) or Hypertrophy (HR) indexes in fast and slow fibers (DM2 n = 13; CTR n = 9).