|
Strains
|
| CH3 |
Riemerella anatipestifer serotype 1 strain, strong biofilm-producer |
[9]
|
|
Escherichia coli S17-1 |
lpir hsdR pro thi; chromosomal integrated RP4-2 Tc::Mu Km::Tn7 |
[32]
|
| BW19851 (pEP4351) |
Plasmid pEP4351 in BW19851, chloramphenicol resistant |
[33]
|
| CH3 (pCP29) |
Riemerella. anatipestifer CH3 strain carrying plasmid pCP29 |
This study |
| BF19 |
Tn4351 insertion mutant of Riemerella. anatipestifer CH3, dhdps::Tn |
This study |
| BF19 (pCP29) |
Mutant BF19 carrying plasmid pCP29 |
This study |
| BF19 (pCP-dhdps) |
Mutant BF19 carrying plasmid pCP29-dhdps |
This study |
|
Plasmids
|
| pEP4351 |
pir-requiring R6K oriV; RP4 oriT; CmrTcr (Emr); vector used for Tn4351 mutagenesis |
[34]
|
| pCP29 |
ColE1 ori; (pCP1 ori); Apr(Emr); E. coli-F. johnsoniae shuttle plasmid |
[33]
|
| pCP-egfp |
pCP29 containing dhdps ORF under the control of the promoter of ORF1, cfxAr (Apr) |
This study |
| pCP-dhdps |
pCP29 containing ompA promoter and dhdps ORF, cfxAr (Apr) |
This study |
|
Primers
|
| 340 |
5'-GACTTGGATACCTCACGCC-3'
|
[35]
|
| 341 |
5'-TTGGAAATTTTCTGGGAGG-3'
|
[35]
|
| TN-1 |
5'-GGACCTACCTCATAGACAA-3'
|
[30]
|
| IS4351-F |
5'-TCAGAGTGAGAGAAAGGG-3'
|
[30]
|
| SP1 |
5'-CTCCCAGAAAATTTCCAAGACTCTCA-3'
|
This study |
| SP2 |
5'-TAAAGTGCTGACCCGTAAAACGAAC-3'
|
This study |
| SP3 |
5'- GTGGTAGCTATAGCATGGAGCTTGC-3') |
This study |
| CAT-1 |
5'-CACTGGATATACCACCG-3'
|
This study |
| CAT-2 |
5'-TGCCACTCATC GCAGTA-3'
|
This study |
| ompA promoter P1 |
5'-CAGGTACCATAGCTAAAATTTTGGCAGTAAC -3' (Kpn I site underlined) |
This study |
| ompA promoter P2 |
5'-CGACTCGAGCATTCCAATTCTCTTATTATC-3' (Xho I site underlined) |
This study |
| dhdps P1 |
5'-TACTCGAGATGAAAAATTTATCAGGTCTAGG -3' (Xho I site underlined) |
This study |
| dhdps P2 |
5'-ATGCATGCTTAACTGAAAACAGAGTGTAGTC-3' (Sph I site underlined) |
This study |
