Skip to main content
. 2012 Jun 29;7(6):e39664. doi: 10.1371/journal.pone.0039664

Figure 2. 2-DE images of total cellular proteins extracted from 6-shogaol-treated SMMC-7721 cells.

Figure 2

(A) SMMC-7721 cells were treated with or without 20 µM 6-shogaol for 24 h. Differentially expressed spots are shown by the arrows. (B) The expanded region of altered protein spots, the proteins with arrow are the differentially expressed proteins identified by MS. (C) The intensity of mitochondria-related and other proteins. (D) The intensity of ER-related proteins. (E) Western bolt imaging of GRP94, GRP78, HSP70 and HSP60. For Western blot analysis, each lane was loaded with 30 µg of protein. Blots were subsequently stripped and reprobed with antibody against β-actin to ensure equivalent loading and transfer. Two additional studies yielded equivalent results.