Figure 6. In vivo antitumor effects of 6-shogaol in SMMC-7721 xenografts.

SCID mice were inoculated with SMMC-7721 cells (3×10⁶ cells/mouse, s.c.) and randomly divided into three groups (8/group) treated with 6-shogaol (10 mg/kg and 50 mg/kg, i.p., daily) or vehicle control solvent. (A) Average tumor volume in vehicle control mice and mice treated with 10 mg/kg and 50 mg/kg 6-shogaol. ** P<0.01. (B) Body weight changes of mice during the 28 days of study. (C) Representative photographs of biopsy samples from mice treated with 6-shogaol (50 mg/kg) and vehicle control. Original magnification ×400. (D) Expression of p-PERK, eIF2α, and p-eIF2α in tumor tissues of SMMC-7721 xenograft mouse model treated with 6-shogaol (50 mg/kg) and vehicle control.