Table 1. Protein alterations in response to 6-shogaol treatment (20 µM for 24 h).

Spots	Protein name	Accession No.	MW(KDa)/pI	Protein score	Sequence coverage%	No. of Queries matched		Fold difference(6-shogoal: Ctrl)
Endoplasmic reticulum
53	Endoplasmin	ENPL_HUMAN	92.7/4.76	392	24		23	1.98±0.23
70	Heat shock protein HSP 90-beta	HS90B_HUMAN	83.5/4.97	454	33		25	2.22±0.29
179	78 kDa glucose-regulated protein	GRP78_HUMAN	72.4/5.07	766	41		40	1.89±0.29
209	Heat shock 70 kDa protein1	HSP71_HUMAN	70.3/5.48	710	43		30	3.04±0.34
436	Protein disulfide-isomerase A6	PDIA6_HUMAN	48.6/4.95	166	22		7	1.52±0.26
778	Calreticulin (grp60)	CALR_HUMAN	48.3/4.29	197	30		10	1.89±0.23
Mitochondria
311	60 kDa heat shock protein	CH60_HUMAN	61.2/5.7	610	45		36	1.87±0.13
424	ATP synthase subunit beta	ATPB_HUMAN	56.5/5.26	316	31		18	3.62±0.22
644	Voltage-dependent anion-selective channelprotein 2	VDAC2_HUMAN	32.1/7.49	139	27		6	–2.73±0.08
Cytoplasm and Others
224	Keratin, type II cytoskeletal 7	K2C7_HUMAN	51.4/5.50	289	24		12	3.66±0.41
274	Keratin, type II cytoskeletal 8	K2C8_HUMAN	53.7/5.52	348	21		12	1.99±0.21
298	T-complex protein 1 subunit zeta	TCPZ_HUMAN	58.5/6.23	165	17		11	–3.59±0.31
509	Keratin, type I cytoskeletal 18	K1C18_HUMAN	48.0/5.34	160	31		15	1.75±0.19
650	Annexin A5	ANXA5_HUMAN	36.0/4.94	357	37		20	2.77±0.22
691	Apolipoprotein A-I	APOA1_HUMAN	30.7/5.56	87	15		3	1.65±0.12