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. Author manuscript; available in PMC: 2012 Dec 28.
Published in final edited form as: Nature. 2012 Jun 28;486(7404):490–495. doi: 10.1038/nature11163

Figure 2. Nutritional regulation of mTORC1 in Paneth cells.

Figure 2

a. Overnight fasted mice treated without or with rapamcyin were refed for 5 hours and intestinal sections immunostained for phospho-S6 (P-S6). Refeeding or administration of insulin activates mTORC1 in Paneth cells (arrow heads) but not ISCs (arrows, n=3–5, scale bar = 20 μm). b. Similar results were observed in cytospun preparations of sorted Paneth cells that were confirmed to be greater than 95% positive for the Paneth cell marker Lysozyme (n=3). P-S6 was reduced in sorted Paneth cells from fasted mice and was induced 20 minutes after injection with insulin. c. Immunoblots of isolated crypts from CR mice show reduced phosphorylation of known mTORC1 substrates phospho-S6K1 (P-S6K1) and P-S6.